ウサギ顎骨感染モデルにおける抗菌剤の治療効果の評価に関する研究 オートバクテリオグラフィーによる観察

Abstract

In order to study the effects of temafloxacin (TMFX), a new quinoline antimicrobial agent, in dentistry and oral and maxillofacial surgery, a jaw bone infection model was prepared in rabbits with Streptococcus millefi (S. milleri) and Bacteroides fragilis (B. fragilis) according to the Satoh-Heimdal method.Both strains were induced to be rifampicillin resistant (RFPr), and the infected rabbit mandible was evaluated by autobacteriography (ABG). In the ABGs, the inoculated bacteria, S. millefi RFPr and B. fragilis RFPr, were largely confined to the infected foci, and low concentrations were observed in the submandibular lymph nodes.TMFX therapy was instituted in both groups at 3 and 7days after infection. In both groups abscesses were more localized and there was a greater decrease in inoculated bacteria compared with the untreated control group. Irrespective of the time of starting treatment, the duration of treatment (3days or 5days) had little effect on therapeutic efficacy evaluated by ABG. However, comparing the results by the time of initiating treatment indicated that treatment should be instituted 3 days after onset of infection when the inflammation was most advanced because an adequate therapeutic effect was obtained. Initiation of treatment at this time also proved beneficial by containing the inflammation and localizing abscesses.The concentration of TMFX in bone tissue was about half of that in serum, and penetration to the lymph nodes and glandular tissue was particularly high.