ВОЗМОЖНОСТИ ПРИМЕНЕНИЯ ПРОТЕОЛИТИЧЕСКИХ ФЕРМЕНТОВ В ЛИМФОТРОПНОЙ ТЕРАПИИ

Abstract

Proteolytic enzymes have a variety of pharmacological effects, which determines their important role and place in clinical medicine. The aim of this study was to confirm the lymphostimulating effect of terrilytin, an integral part of the drug terridecase, and to study its effect on the pharmacokinetic parameters of cefotaxime. Materials and methods of research. Assessment of the rate of lymphatic drainage in the mesentery of mice by the time of removal of lymphotropic dye from it; determination of the concentration of the antibiotic cephalosporin antibiotic of the third generation cefotaxime in rabbit blood plasma during the day, blood plasma, intestinal tissues and liver of white mongrel mice after 1.5 and 24 hours of experiment by high-performance liquid chromatography. Results. It has been confirmed that terrilytin has a lymphostimulating effect. It has been established that this proteolytic enzyme has the properties of an endolymphatic conductor. Its preliminary administration increases the concentration of cefotaxime in the blood plasma of rabbits and mice compared with monovision of the antibiotic at all time points of the study, up to a day, and increases its content in the intestinal tissue of mice, both 1.5 hours and 24 hours after injection of drugs. Terrilytin does not affect the level of cefotaxime in the liver tissue of mice, but reduces the ratio of its concentrations "liver tissue/blood", which indirectly indicates a decrease in liver extraction of the antibiotic. Conclusion. Terrilytin in clinical practice was used topically (externally or electrophoretically), whereas our studies were conducted against the background of its parenteral administration. This obstacle can be overcome by using a polyglucine–modified form of terrilytin – terridecase, which is allowed for parenteral administration. In this regard, it is advisable to continue studying the combined drug terridecase in order to identify its ability to direct water-soluble antibiotics into the lymphatic system.