Abstract
To understand the effect of reactive oxygen species (ROS) on floral senescence in Hibiscus syriacus L., we have investigated change in relative water potential, malondialdehyde (MDA) content, <TEX>H_2O_2</TEX> content and the activity of antioxidative enzymes in the petals during flower opening and senescence. Hibiscus flowers were achieved full bloom at early morning and started to in-rolling and showed petal in-rolling over than 50% at 24 h and 36 h after full bloom, respectively. The flower was a decrease in fresh weight by 30% and showed water loss with floral senescence. MDA content and activity of antioxidative enzymes such as APX, GR and CAT were showed no significant change until 36 h after full bloom. In the flower 48 h after full bloom that showed complete petal in-rolling and wilting, however, activity of antioxidative enzymes and <TEX>H_2O_2</TEX> content was greatly increased as compared with 0 h after full bloom. These results suggest that reactive oxygen species are related to accelerating the later senescence more than inducing the early senescence during Hibiscus flower senescence.
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