環境中有機塩素化合物の相互作用の解析 ラット胎仔肢芽培養法を用いて

Abstract

Interactions of binary mixutues among organochlorine compounds detected in the water and sediment of urban rivers were analysed in cultured limb bud cells using Toxic Unit analysis and isobole diagram.Type and mode of action of mixtures vary depedns on the mixture ratio and end points (chondrogenesis, cell proliferation) which resulted in varied potential of developmental toxicity among mixtures.As limb bud cell culture (LBC) assesses cell differentiation and cell proliferation simultaneously, LBC combined with Toxic Unit analysis and isobole diagram provides useful tool for analysis of interation of mixture.