좌골신경손상 기간에 따른 근섬유별 위축 및 관련 유전자 발현에 미치는 영향

Abstract

In order to analyze how the protein synthesis and degradation gene expression changes by muscle fiber type depending on the degree of atrophy after sciatic nerve injury, this study was conducted to analyze the difference of atrophy-related genes according to the recovery period after damaging the left sciatic nerve of the white rats. For this purpose, 16 Spargue-Dawley male rats were randomly divided in sham, 5days, and 10day groups and the expression of MyoD, Myogenin, MuRF1 and MAFbx was analyzed in gastrocnemius, extensor digitorum longus, soleus. BR The Sciatic nerve injury group decreased in weight as time elapsed, but sham group decreased and recovered. Dietary intake decreased in both groups and then increased again and given that there is no difference in dietary intake among groups, dietary intake is thought to have no effect on body weight and skeletal muscle weight.BR The skeletal muscle weights were significantly different between the three groups due to the difference in injury duration in all three muscles, Type I soleus is considered to be significantly contracted than Type II extensor digitorum longus muscle due to Sciatic nerve injury.BR The expression of MyoD, Myogenin, MuRF1, and MAFbx in gastrocnemius, extensor digitorum longus muscle, soleus contracted with scatic nerve injury was increased compared to the sham group. In addition, the expression of MyoD was found to be higher in extensor digitorum longus muscle composed of Type II fibers, and that of Myogenin was higher in soleus composed of Type I fibers. The expression of MuRF1 was mainly high in extensor digitorum longus muscle, and that of MAFbx was mainly high in gastrocnemius and soleus.