반응표면분석법을 이용한 인진쑥 유효성분의 에탄올 추출조건 최적화

Abstract

This study was conducted to monitor the quality characteristics of Artemisis capillaris ethanolic extract by response surface methodology. The independent variables were extraction temperature (X1; 60, 70, 80, 90, and 100°C), extraction time (X2; 1, 2, 3, 4, and 5 hr), and ethanol concentration (X3; 0, 20, 40, 60, and 80%). Soluble solid content (Y1), chlorogenic acid content (Y2), and coumaric acid content (Y3), etc. were analyzed as the dependent variables. Estimated optimal conditions for soluble solids were an extraction temperature of 87.65°C, extraction time of 3.19 hr, and ethanol concentration of 42.40%. The optimal extraction conditions for chlorogenic acid were 84.30°C, 3.14 hr, and 47.85%, respectively. Further, those for coumaric acid were 83.45°C, 3.40 hr, and 45.39%, respectively. Extraction conditions for effective components of Artemisis capillaris were superimposed by response surface plots on optimization extraction condition of each dependent variable, including soluble solid, chlorogenic acid, and coumaric acid contents. As a result, superimposed extraction conditions were 80~90°C, 3~4 hr, and 40~50%, respectively. Under these conditions, soluble solid, chlorogenic acid, and coumaric acid contents were 1.09%, 25.66 mg%, and 20.25 mg%, respectively.