Abstract

Brassica spp. vegetables have been known to have biological activities such as anti-cancer and anti-inflammatory activities. In this study, we investigated the in vitro physicochemical characteristics and antioxidant activities of commonly used Brassica spp. sprout vegetables such as kohlabi (Brassica oleracea var. gongyloides), red radish (RaphanussativusL. var. sativus), broccoli (B. oleraceavar. italica), cabbage (B. rapavar. glabra Regel), rape (B. napus), radish (R. sativus), and tatsoi (B. campestris var. narinosa) sprouts. Our results showed that the vegetables with the highest total phenolics contents were the radish sprout (24.40±1.24 mg TAE/g) and kohlabi sprout extracts (23.97±0.46 mg TAE/g). Furthermore, the vegetable with the highest total flavonoid content was the radish sprout extract (15.30±1.35 mg CE/g). However, the kohlabi sprout extract showed the highest DPPH radical scavenging value (IC50= 1.95 mg/mL) and ORAC value (79.03 mM TE/g). In addition,the six kinds of Brassica spp. sprout vegetable extracts, except tatsoi, significantly inhibited the reactive oxygen species (ROS) production and showed thatintracellular oxidative stress is closely related tothe accumulation of differentiated adipocytes and fat during the adipogenesis of 3T3-L1 preadipocytes. These results suggest that Brassica spp. sprout vegetables, especially kohlabi and radish sprout extracts, can be used to develop natural antioxidants.

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