Abstract
This study was carried out to establish the optimum condition of cell disruption with a sonicator for the detection of the spore, Bacillus anthracis <TEX>${\Delta}$</TEX>-sterne for the purpose of developing automatic fluorometer. The efficiency of sonication on the <TEX>${\Delta}$</TEX>-sterne spore disruption was very weak. The <TEX>${\Delta}$</TEX>-sterne spore with zirconia bead showed greater disruption than the <TEX>${\Delta}$</TEX>-sterne spore alone when sonificated. The volumn of the zirconia bead added in the spore solution has little effect on the disruption efficiency. The detection limit of the <TEX>${\Delta}$</TEX>-sterne spore with zirconia bead and the <TEX>${\Delta}$</TEX>-sterne spore alone was <TEX>$10^6$</TEX> CFU/mL and <TEX>$5{\times}10^7$</TEX> CFU/mL respectively, when sample was sonicated for 20 seconds with a sonicator probe of 13 mm diameter.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
