АККУМУЛЯЦИЯ В ПОЧКЕ ЭКЗОГЕННОГО БЕЛКА ПОСЛЕ ЕГО ВСАСЫВАНИЯ В КИШЕЧНИКЕ ПРИ РАЗВИТИИ ЭКСПЕРИМЕНТАЛЬНОЙ ПОЧЕЧНОЙ НЕДОСТАТОЧНОСТИ У КРЫС

Abstract

THE AIM of the investigation was to study the possibilities of accumulation of the green fluorescent protein (GFP) in the rat kidney under conditions of experimental chronic renal failure (CRF). MATERIALS AND METHODS. The experimental group consisted of 13 Wistar rats subjected to subtotal nephrectomy (SNE). In eight days after operation GFP was introduced to the animals through stomach probe. The control group consisted of 12 rats with intact kidneys. The concentration of creatinine, blood plasma urea, diurnal diuresis, concentration of albumin and urine creatinine with calculation of the albumine/cratinine ratio (ACR), concentration index (Ucr/Pcr) and creatinine clearance (Ccr ) were determined in both groups. In addition, light-optical and electron-microscopic investigations were carried on. Confocal microscopy was used for the assessment of accumulation of GFP in the erythrocytes andcell populations of the nephron. RESULTS. It has been found that GFP is absorbed by erythrocytes and, on coming into the systemic circulation, is accumulated in the cell vesicles of the proximal tubules (PT). Morphological and functional changes in the rats with the developing renal failure after SNE are followed by a considerable decrease of accumulation of GFP in PT as compared with the animals without dysfunction of the kidneys. CONCLUSION. The example of the model with GFP in animals with experimental CRF for the first time has demonstrated the fundamental possibility of enteral transport and accumulation of intact foreign proteins in the cells of the proximal nephron segment. The data obtained open new perspectives for studying the role of the kidneys in metabolism of exogenous proteins.