Abstract
경골어류 4종(조피볼락, 용치놀래기, 송곳니베도라치 및 졸복) 장관의 복합당질 성상의 차이를 밝히기 위해 장을 네 부위로 나누워 아홉 종류의 biotinylated lectin들(DBA, SBA, PNA, BSL- I , RCA- I , sWGA, UEA- I 및 Con A)로 반응시켰다. 조피볼락을 제외하면 부위에 따른 차이는 없었다. 용치놀래기 장 상피는 모든 부위에서 D-glucose/mannose 잔기를 함유하고 있었다. 조피볼락, 송곳니베도라치 및 졸복 장 모든 부위에서는 정도의 차이는 있지만 <TEX>${\beta}$</TEX>-N-acetyl-D-gal-actosamine가 함유되어 있었다. 또한 조피볼락의 근위장, 중간장 및 원위장에는 galactose-<TEX>${\beta}$</TEX>-1,3-N-acetyl-D-galactosamine 및 <TEX>${\alpha}$</TEX>-D-galactose도 함유되어 있었으나 직장에는 이들 당 잔기들이 함유되어 있지 않았다. 송곳니베도라치에는 <TEX>${\beta}$</TEX>-N- acetyl-D-galactosamine 외에 <TEX>${\beta}$</TEX>-N-acetyl-D-glucosamine 및 D-glucose/mannose도 발견되었으며, 졸복에서는 Galactose-<TEX>${\beta}$</TEX>-1,3-N-acetyl-D-galactosamine, D-galactose 및 D-glucose/mannose도 발견되었다. The aim of this work is to show differences in the pattern of glycoconjugate composition in the intestines of four teleostean species (Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus, and Takifugu pardalis). We compared four regions of all species studied. The specimens were processed and stained with nine kinds of biotinylated lectins (DBA, SBA, PNA, BSL- I , RCA- I , sWGA, UEA- I , LCA and Con A). Except for Sebastes schlegeli, no differences between regions were observed. The intestinal epithelium of Halichoeres poecilopterus possessed D-glucose/mannose residues in all regions. <TEX>${\beta}$</TEX>-N-acetyl-D-galactosamine was distinctive along the intestines, although the pattern of diversity was different in Sebastes schlegeli, Bryzoichthys lysimus, and Takifugu pardalis. Additionally, the occurrence of Galactose-<TEX>${\beta}$</TEX>-1,3-N-acetyl-D-galactosamine and <TEX>${\alpha}$</TEX>-D-galactose were confirmed in the proximal, middle, and distal intestine of Sebastes schlegeli, while rectal intestine lacked these sugar residues. Along with <TEX>${\beta}$</TEX>-N-acetyl-D-galactosamine, <TEX>${\beta}$</TEX>-N-acetyl-D-glucosamine and D-glucose/mannose were also determined in Bryzoichthys lysimus. Galactose-<TEX>${\beta}$</TEX>-1,3-N-acetyl-D-galactosamine, D-galactose, and D-glucose/mannose were also present in Takifugu pardalis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.