簡単なおこなえる血小板製剤の外観試験法 ｓｗｉｒｌｉｎｇ ｐａｔｔｅｒｎ ｔｅｓｔ．

Abstract

The normal viable platelets display a “streaming or swirling pattern” when the container is agitated under the appropriate lighting condition. The swirling patterns of platelet concentrates (PC) gradually disappeared with lowering pH of PC stored for 3 to 4 days. The change in platelet morphology from disc from to sphere forms was also closely related with decreasing pH of PC. We classified the swirling patterns into two categories, (-) or (+), or into three categories, (-), (+), or (++). PC with a pH level of 6.0-6.2 had a score of (-) in the 2 categories classification. The pH of PC with a score of (+) ranged from 6.2 to 7.1. In the 3 categories classification PC with a pH of 6.2-6.8 had a score of (+) and pH of PC with the score of (++) was 6.8-7.1. In PC with the score of (-) platelet swelling and decreased aggregation responses to collagen were apparent. Red cell contamination did not affect seriously the evaluation of swirling patterns. Now we have obtained a simple and easy method utilizing swirling patterns of platelets for the non-invasive evaluation of the quality of stored platelets.