タール色素のＲＮＡ合成反応に及ぼす影響 ＩＩ タール色素のラット肝臓単離核およびラット肝臓ＲＮＡポリメラーゼ粗酵素のＲＮＡ合成反応に及ぼす影響

Abstract

Synthetic food dyes were tested for their effects on RNA synthesizing activity in nuclei isolated from rat liver and DNA dependent RNA polymerase partially purified from isolated rat liver nuclei.1) Azo dyes (amaranth, ponceau R, ponceau 3R and orange I) highly inhibited the RNA polymerase reaction partially purified enzyme at a concentration of 1 mM.In contrast with partially purified enzyme, amaranth, ponceau R and ponceau 311, stimulated RNA synthesis in isolated rat liver nuclei. These dyes were assumed to stimulate DNA like RNA synthesis in nucleoplasm, because the stimulation of RNA synthesis by these dyes was dependent on Mn2+ ion.2) Xanthene dyes (rose bengale, phloxine, erythrosine and eosine) highly inhibited the RNA polymerase reaction of partially purified enzyme, but acid red little effect. The results suggest that halogen atom is essential to the inhibition of RNA synthesis by xanthene dyes.Rose bengale and phloxine inhibited the RNA synthesizing reaction in rat liver nuclei at a concentration of 1 mM.3) Triphenylmethane dyes (light green SF, acid violet 6B, brilliant blue FCF and fast green FCF) inhibited the RNA polymerase reaction of partially purified enzyme.Light green SF stimulated RNA synthesis in isolated nuclei and the stimulating activity was also dependent on Mn2+ ion.4) Indigo carmine gave no effect on both RNA synthesizing activities.