Сравнительный гистологический анализ лоскутов крайней плоти и слизистой щеки для лечения проксимальных форм гипоспадии у детей

Abstract

Buccal mucosa and preputial skin grafts are used for staged urethroplasty in proximal forms of hypospadias in children. Aim of our study was to carry out a comparative histological analysis of preputial skin and buccal mucosa. s and materials: Histological analysis of urethral tissue samples from 10 patients with proximal forms of hypospadias was conducted. All patients were treated with staged Brackas technique using free grafts at the Russian Childrens Clinical Hospital from 2013 to 2016. Patients were divided into two groups. In Group I (n=5), preputial skin graft was used for urethroplasty, while in Group II (n=5) buccal mucosa was taken. A histological study of both materials with comparison to a native urethra was performed. In Group I, the histological analysis showed keratinizing multilayered squamous epithelium, large number of dilated sweat and sebaceous glands with signs of inflammation, and hair follicles. In Group II, histological analysis revealed the presence of the typical structure for mucosal tissue, including multilayered flat non-keratinizing epithelium, as well as full absence of sweat, sebaceous glands, and hair follicles. comparative histological analysis of neourethra has shown the absence of sweat and sebaceous glands, hair follicles, and areas of chronic inflammation in buccal mucosa vs preputial skin, showing that buccal mucosa is more similar to native urethra. Therefore, buccal mucosa is favored as the material for urethroplasty.