Генетична структура сазана амурського ТзОВ «Карпатський водограй»

Abstract

The purpose. To study genetic structure of breed herd of Amur carp selected in «Karpatskyi vodohrai» Ltd. (Lviv oblast) with the use of different types of markers and to determine the level of somatic mutagenesis by the microkernel test. Methods. Laboratory researches, computer statistical analysis. Results. Genetic structure of Amur carp on separate types of molecular-genetic markers (DNA-markers, genetic-biochemical systems) is studied. Species-specific features of genetic structure on probed loci are determined. The level of actual and expected heterozygosity is calculated. Increased allele and genotypic variety of genetic structure can be caused by a little heightened intensity of the carried out selection work. The detected excess of heterozygotes on separate loci testifies to presence of stabilization processes of genetic structure. Research of the level of somatic mutagenesis is also carried out at use of micronucleus test. Conclusions. At use of different types of molecular-genetic markers the information on genetic structure of a carp and its variety on genome level is gained. These results testify to the following: genome preserved resistant gene complexes, despite of significant selection effort. The applied genetically-statistical approaches can be used for monitoring genetic structure of groups of a carp of Ukraine, determination of the level of consolidation and phylogenetic links between them with their subsequent genetic certification. Results of researches of the level of somatic mutagenesis at use of micronucleus test shown that the probed group of fishes was characterized by average level of erythrocytes with micronucleus MNE (4,7±0,3‰), low level of lymphocytes with micronucleus and binucleate lymphocytes which total has made 3,5±0,3‰ that in its turn testifies to satisfactory conditions of selection.