Abstract
Some kinds of phospholipids have been detected as extracellular substance during the culture of Thiobacillus thiooxidans and Thiobacillus ferrooxidans, and during the pyrite leaching by Thiobacillus ferrooxidans. In this paper, the function of phospholipid in bacterial leaching of chalcopyrite concentrate by Thiobacillus ferrooxidans was investigated, using phosphatidylglycerol as a phospholipid.Phosphatidylglycerol was observed to be adsorbed on chalcopyrite surface. As ferric ions would be inhibited to react with the chalcopyrite due to the adsorbed layer of phosphatidylglycerol, the amount of copper chemically leached with ferric sulfate become small with increasing phosphatidylglycerol addition. In the medium containing ferrous sulfate as substrate, growth of Thiobacillus ferrooxidans and iron oxidation from ferrous to ferric by the microorganisms were delayed with phosphatidylglycerol addition. On bacterial leaching, copper extraction was decreased with increasing phosphatidylglycerol addition. The leaching results were interpreted in terms of the functions of phosphatidylglycerol, which influenced the main reaction of indirect leaching mechanism.
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