Abstract

Currently, a new generation of immunomodulators, cytokines, are widely used in medicine and veterinary medicine. Cytokines are active in very low concentrations, they regulate the proliferation and differentiation of cells of the immune system. Taking into account the high biological activity for cells of animal, plant and microbial origin in vivo and in vitro, we conducted the present studies, the purpose of which was to study the possibility of using cytokines as activators of the metabolism of animal cell cultures in vitro for the reproduction of viruses on them. In the experiments, cell cultures MDBK and BHK-21/13-02 obtained from the collection of cell cultures of the Federal State Budget Scientific Institution FSBSI «FCTRBS-ARRVI» were used. Nutrient media were used for growing cell cultures: Needle MEM, solution of Versen, trypsin, Hanks, bovine blood serum (BRS), fetal blood serum (FBMS). As activators of cell metabolism, commercial cytokines were used: IL-3, IL-6, colon-stimulating factor G-CSF produced by Cytokin LLC (St. Petersburg). Stimulating and inhibitory doses of cytokines were experimentally determined and added to media with cell cultures at the rate of 30 to 500 pg/cm3. It was found that among the tested classes of cytokines, interleukin-6 (IL-6) turned out to be the most active, which, when introduced into the growth medium at a concentration of 30-60 ng/cm3, had a metabolism-stimulating effect, providing a proliferation index of MDBK cells by 1.33 times and cell lines VNK-21/13-02 - 1.17 times, respectively.

Full Text

Submitted Version (Free)

Published Version

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.