Abstract
Hydrolysis of triolein catalyzed by Rhizopus delemar lipase was investigated as a function of the water and AOT concentrations in AOT reversed microemulsion systems. In this study, the W (= [H2O] / [AOT]) value was used as a parameter of the water concentration. The initial reaction rate remained constant at W of 10-12. In the fixed W systems, the reaction rate apparently increased with increasing the AOT concentration. The conversion yield after 24 h showed a maximum at an AOT concentration of 50 mM and W of 10. The maximum velocity depended on both the W value and the AOT concentration. Its change corresponded to the profile of the initial reaction rate. The Michaelis constant was found to be the same for the value of W larger than 12, and it was independent of AOT concentration.
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