Abstract
Whole blood (400ml) was collected in citrate-phosphate dextrose in quodruple bag system. After centrifugation for 6min at 2, 200rpm, blood was separated into platelet rich plasma, huffy coat (approximately 45ml), and concentrated red cells. The platelet rich plasma was separated into platelet concentrates and plasma. The buffy coat contained approximately 98% lymphocytes, 70% granulocytes and 12% red cells of the original value. The leukocyte contents of huffy-coat-depleted concentrated red cells were 50±28×107per unit, in particular, lymphocytes decreased to 1.4±0.5×107per unit, because of the most lymphocytes were removed with buffy coat. This system seems to be as safe and effective for preparation of leukocytes poor red cells, and can be operated without machines.
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