ФОТОДИНАМИЧЕСКАЯ ТЕРАПИЯ ЭКСПЕРИМЕНТАЛЬНОГО ХОЛАНГИТА

Abstract

Background. Photodynamic therapy of acute cholangitis involves the introduction of a photosensitizer into the common bile duct and subsequent laser irradiation of the liver. The study of combined laser action on the liver is promising. Purpose of the study. To evaluate in an experiment the effectiveness of photodynamic therapy of acute cholangitis with the introduction of a photosensitizer into the common bile duct and combined laser irradiation of the liver. Material and methods. Acute cholangitis was modeled in rabbits. In the first group, a saline solution was introduced into the common bile duct, in the second and third – "Photolon". Then, in the second group, photodynamic therapy with endocholedocheal laser irradiation of the liver was performed, in the third group – with combined irradiation. The general condition of the animals, their laboratory, microscopic and microbiological parameters were evaluated. After the autopsy, a histological examination of the liver and common bile duct was performed. Results. On the 2nd day of the experiment, all rabbits developed acute cholangitis. After treatment in the group "Experience 3", in comparison with other groups, there was a greater decrease in the indicators of inflammation and cholestasis in the blood, the number of microorganisms and white blood cells in the bile. In the first group, the formation of liver abscesses was histologically noted. Pathological changes in the wall of the common bile duct and liver in the third group were less pronounced than in the second. Conclusions. 1. The experimental model allows on the second day to reproduce acute cholangitis in rabbits. 2. Photodynamic therapy with Photolon photosensitizer with combined (endocholedocheal and surface) laser irradiation of the liver has a more pronounced bactericidal effect compared to endocholedocheal irradiation.