包括固定化Ｐｓｅｕｄｏｍｏｎａｓ ａｅｒｕｇｉｎｏｓａによるジベンゾフランの生分解

Abstract

In resent years, some of dioxin congeners have been detected in landfill leachate and drainage of paper mill and causing serious social problems. Appropriate methods for treating these congeners are urgently needed.This study was undertaken to develope the method to biodegrade the dioxin congeners using immobilized Pseudomonas aeruginosa which is able to utilize specifically dibenzofuran (DBF), fundamental element of dioxin. As an immobilizing chemical, polyethyleneglycol, polyacrylamide and sodium alginate were tested. The pellets immobilized with sodium alginate were unsuitable for using as the immobilizing agent because they dissolved extensively in a growth medium. The optimum temperature for degradation of DBF was found to be 30°C and the optimum pH was about 7. The biodegradation of DBF was significantly enhanced in the presence of landfill leachate.In a long-term batch cultivation system, it was made clear that the physical strength of the pellet was maintained and the degradation activities of the immobilized microorganisms remained high. In a continuous cultivation system, DBF was degraded as efficiently as in the batch cultivation system.