Abstract

With the cellular technology of keeping laying hens, their mobility is limited. This can lead to various negative consequences due to the development of a protective and adaptive reaction in the body of an agricultural bird. One of the approaches to the early diagnosis of stress reactions in poultry is the study of biochemical parameters of blood serum. Biochemical indicators can provide information about the general condition of the bird, changes in its body and stress levels. In particular, the study of biochemical parameters of blood serum of laying hens at different levels of planting density can help to identify the relationship between the conditions of poultry keeping and its stress response. Mathematical calculations of the estimation of the correlation dependence of biochemical parameters of blood serum of laying hens depending on different levels of planting density with cellular content are important in the interpretation of the data obtained. Fischer's F-criterion (F-distribution) was used for mathematical processing of the research results. The results obtained allow us to conclude that the indicators of cholesterol and total protein in laying hens do not depend on the planting density. Alkaline phosphatase, phosphorus, calcium, ACTH depend on the density of laying hens (the null hypothesis is refuted at 1% significance level). Blood glucose and cortisol levels are also dependent on planting density. Here, the null hypothesis is refuted at the 5% significance level. The results of the study of the levels of alkaline phosphatase, glucose, phosphorus, calcium, adrenocorticotropic hormone (ACTH) and cortisol at different levels of planting density indicate the development of a stress reaction in laying hens and are important diagnostic markers when exposed to technological stresses.

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