Abstract
Organic wastes such as food waste (FW), livestock wastewater (LW), and sewage sludge (SWS) can produce hydrogen (<TEX>$H_2$</TEX>) by anaerobic acid fermentation. Expecially, FW which has high carbohydrate content produces <TEX>$H_2$</TEX> and short chain fatty acids by indigenous <TEX>$H_2$</TEX> producing microorganisms without adding inoculum, however <TEX>$H_2$</TEX> production rate (HPR) and yield have to be improved to use a commercially available technology. In this study, LW was mixed to FW in different ratios (on chemical oxygen demand (COD) basis) as an auxiliary substrate. The mixture of FW and LW was pretreated at pH 2 using 6 N HCl for 12 h and then fermented at <TEX>$37^{\circ}C$</TEX> for 28 h. HPR of FW, 254 mL <TEX>$H_2/L/h$</TEX>, was increased with the addition of LW, however, mixing ratio of LW to FW was reversely related to HPR, exhibiting HPR of 737, 733, 599, and 389 mL <TEX>$H_2/L/h$</TEX> at the ratio of FW:LW=10:1, 10:2, 10:3, and 10:4 on COD basis, respectively. Maximum HPR and <TEX>$H_2$</TEX> production yield of 737 <TEX>$H_2/L/h$</TEX> and 1.74 mol <TEX>$H_2/mol$</TEX> hexoseadded were obtained respectively at the ratio of FW:LW=10:1. Butyrate was the main organic acid produced and propionate was not detected throughout the experiment.
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