Влияние активации ванилоидных рецепторов эводиамином на содержание кальцитонин-ген-родственного пептида в крови крыс в норме и при гипертензии, вызванной потреблением фруктозы

Abstract

Background: Calcitonin-gene-related peptide (CGRP) is a neurotransmitter of capsaicin-sensitive nerves, one of the most powerful vasodilators, in mediation of vasoactive action of which the nitrogen oxide (NO) takes part. The aim of this study was to evaluate the CGRP and NO blood levels in normal rats and rats with fructose-induced hypertension, with unaffected transient receptor potential vanilloid 1 (TRPV1) receptors, and after the administration of their agonist evodiamine. Male Wistar Rats were given to drink either 12.5% fructose solution or tap water (control) for 10 weeks. Evodiamine (10 mg/kg), a TRPV1 agonist, was administered intraperitoneally every other day, for 2 weeks, starting 8 weeks after the start of the fructose intake. The systolic blood pressure was measured using a noninvasive technuque. The glucose levels, fasting and 1 hour after the glucose load (2g/kg, i.p), were measured by a glucometer in the blood sampled from the caudal vein. Concentrations of CGRP, NO metabolites (NOx), triglycerides (TG) and lipid peroxidation products (LPO) were measured in the blood serum of rats. The fructose consumption led to hypertension, impaired glucose tolerance, increased the blood levels of LPO products and TG, and decreased the CGRP blood content. The evodiamine introduction to fructose-consuming rats restored the blood CGRP content to the control values, reduced the blood pressure, LPO products and TG levels, and improved the glucose tolerance. The NOx levels did not differ from the control in all groups. We suggest that CGRP is involved in the development of the fructose-induced hypertension and in mediating the therapeutic effect of the evodiamine.