Элементы технологии производства микробиопрепаратов на основе перспективных бактериальных штаммов антагонистов возбудителя сухой гнили подсолнечника

Abstract

The elements of production technologies of microbiopreparations in a preparative form wetting powder at a stationary surface cultivation of promising bacterial antagonist strains of a dry rot pathogen on sunflower. The optimal sources of nitrogen and carbon-based and compound nutrient media for cultivation of three promising bacterial strains from the genera Bacillus and Pseudomonas. An excellent source of nitrogen for a strain 5B-1 Bacillus subtilis is corn steep liquor, and of carbon – glycerin and molasses. The highest weight of dry bacterial biomass of a strain D 1-1 Bacillus sp. was on a nutrient medium where sodium nitrogen was used as a nitrogen source and sucrose and molasses – as a carbon-based source. For both bacterial strains of a genus Bacillus the optimal were such compound nutrient media as Chapek’s for bacteria and Tylon-3. For cultivation of a strain 14-4 Pseudomonas sp. the best nitrogen sources were corn steep liquor and sodium nitrogen, and carbon-based – sucrose. The most optimal compound nutrient medium for these strains were King B and Chapek’s for bacteria.