Abstract
The appearance of precipitates and opalescent components hinders testing for the sterility of insulin suspensions prior to seeding in cultural media. The sampling procedure is improved by dissolving preparations in 0.2% ascorbic acid solution, which eliminates opalescence and precipitation and facilitates the reading and interpretation of results as the test solutions become transparent. This method is suitable with both direct inoculation and membrane filtration. The insulin solution in 2% ascorbic acid does not exhibit antimicrobial activity, which makes expedient the testing with more economic direct inoculation.
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