РАЗРАБОТКА ТЕХНОЛОГИИ ЭКСТРАКЦИОННОГО ПРЕПАРАТА ЛЕСПЕДЕЦЫ ДВУХЦВЕТНОЙ И ИЗУЧЕНИЕ ЕГО ПРОТИВОВОСПАЛИТЕЛЬНОЙ АКТИВНОСТИ В КОМБИНАЦИИ СО СТРЕПТОЦИДОМ

Abstract

The article presents the results of the development of a rational method for obtaining an extraction preparation of shoots of Lespedeza bicolor, the results of determining the indicators of authenticity and quality in it, and the results of studying the anti-inflammatory effect. The extract preparation of shoots was obtained according to the well-known technological scheme: extraction of raw materials, purification of the extract, thickening of the extract. Ethyl alcohol of 70% concentration was chosen as the extractant, which is associated with the extraction of alcohol of this concentration of flavonoids - active biologically active substances of the raw material. The finished product was a thick liquid, greenish-brown in color with a specific odor. The yield of dry residue in the thick extract of shoots of Lespedeza bicolor was 22,2 ± 5,78%, the content of flavonoids, in terms of quercetin, was 1,98±0,027%. To study the local anti-inflammatory effect of the extract of the shoots of Lespedeza bicolor, studies were carried out to develop the composition and biopharmaceutical evaluation of a soft dosage form - an ointment with an extract of the shoots of Lespedeza bicolor. When biopharmaceutical evaluation of the release of the resulting ointment (gel) by diffusion through a semi-permeable membrane into an aqueous medium, it was determined that the maximum release of biologically active substances (flavonoids) from the gel is observed after 10 minutes. The introduction of an additional antimicrobial component, streptocide (sulfanilamide), into the composition of the developed gel with an extract of Lespedeza bicolor is substantiated. The study of the anti-inflammatory activity of gels with an extract of Lespedeza bicolor and gels of its combined form with streptocide was carried out on a model of acute exudative inflammation (formalin edema). It has been established that both the gel with the extract of the shoots of Lespedeza bicolor and the gel, containing the combination of the extract of the shoots of Lespedeza bicolor with streptocide, have been found to have anti-inflammatory activity; while the first exceeds the second gel in its activity, inhibiting inflammation more pronounced at the 3rd hour and after 24 hours, and is also more effective than the official drug - indomethacin ointment by 1.2-1.5 times, while remaining less toxic.