比較體內 ，體外二階段、三階段方法及動態模擬系統評估豬飼糧營養成分之消化率

Abstract

The aims of this study was (1) to assess the ability of two-step, three-step in vitro, and dynamic in vitro model compared with in vivo total tract digestibility of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), and starch in pig diets. (2) to compare the digestibility of nutrients in feedstuffs using different methods by in vitro. Using two-step, three-step in vitro, and dynamic in vitro model to estimate digestibilities of nutrients in seven feedstuffs and 5 pig diets were conducted. For diets, the two-step and three-step methods showed higher (P<0.001) DM digestibility than dynamic model and in vivo. However, DM digestibility in dynamic model was no significant different from in vivo. All in vitro methods, the digestibility of CP was higher than the in vivo digestibility of CP. Digestibilities of starch and NDF in three-step method were no significant difference compared to the in vivo. The relationship (R2) between the two-step method and in vivo results for starch and CP digestibilities were 0.92 and 0.82, respectively. The R2 between the three-step method and in vivo results for NDF digestibility were 0.94. While, the R2 between the dynamic model and in vivo results for CP digestibility was 0.95. For feedstuffs, the digestibilities of DM and starch by three-step in vitro method were higher than that by two-step in vitro and dynamic model results. Nevertheless, there were no significant differences in the digestibility of CP among different in vitro methods. Accordingly the results, we can conclude that the two-step and three-step in vitro methods can validly to estimate the in vivo digestibility of starch. Moreover, using in vitro method by three-step to estimated in vivo digestibility of NDF. Whereas, using dynamic model can be to estimated in vivo digestibilities of DM and CP.