慢性辺縁性歯周炎罹患者の血清, 歯肉および歯周ポケット滲出液中のトリグリセライドについて

Abstract

RABINOWITZ et al., YAMAGUCHI and MURAI reported on tri-glycerid in human serum and gingiva. So far as the author knows, there have been no reports on it in gingival fluid.Therefore, in the present study, the author investigated the identification of it in human serum, gingiva and gingival fluid, and the measurement of content of it in them, that occurred in association with the progress of chronic marginal periodontitis.A study material consisted of 76 males and 80 females, ranging from 19 to 72 in age, who were diagnosed as the periodontitis in the department of periodontics, Nihon University Dental Hospital. And it was concluced from carefully clinical examination that each patient was free from disease except the periodontitis.Lipids in human serum, gingiva and gingival fluid were extracted by the method (FOLCH et al's, 1957), evaporated and isolated by the use of thin-layer chromatography. The amount of tri-glycerid in them was determined by the enzyme method-the end point method (reagents: BOEHRINGER MANNHEIM) using the ultra-violet absorption spectrophotometry.When examined in different degrees of chronic marginal periodontitis (P→M→A), the author, as a result, obtained some findings as follows:1. The values of tri-glycerid in human serum revealed a tendancy of increase in consequence of the disease.2. The level of tri-glycerid in human gingiva was observed to decrease in proportion to the aggravation of the disease. In other words, the difference between Group Normal and Group P, and Group M, and Group A was statistically highly significant (P<0.01). The difference between Group P and Group M was statistically significant (P<0.05). And the difference between Group P and Group A, Group M and Group A was statistically highly significant (P<0.01).3. The existence of tri-glycerid was identified in human gingival fluid.4. The level of tri-glycerid in human gingival fluid was observed to increase in proportion to the aggravation of the disease. That is, each difference among Group P, Group M and Group A was statistically highly