Микробиота плодных оболочек при интактном плодном пузыре и доношенной беременности

Abstract

Aim of research. To study the microbial landscape of intact fetal membranes in full-term pregnancy. Materials and methods. In 19 pregnant women (mean age — 31.0 ± 5.3 years, mean gestational age — 39.3 ± 0.65 weeks) with intact fetal membranes, the fetal membrane tissue was collected during elective cesarean section to detect by polymerase chain reaction the following microorganisms: Lactobacillus spp., Enterobacteriaceae, Streptococcus spp., Staphylococcus spp., Gardnerella vaginalis/Prevotella bivia/Porphyromonas spp., Eubacterium spp., Sneathia spp./Leptotrihia spp./ Fusobacterium spp., Megasphaera spp./Veillonella spp./ Dialister spp., Lachnobacterium spp./Clostridium spp., Mobiluncus spp./Corynebacterium spp., Peptostreptococcus spp., Atopobium vaginae, Mycoplasma hominis, Ureaplasma (urealyticum + parvum), Candida spp., Mycoplasma genitalium. Results. Sterile membranes were found in 5 pregnant women (26.3%), in the remaining cases, the total bacterial load (TBL) was 104.5 (103.5–105.8) genome equivalents (GE) per sample. Representatives of the Enterobacteriaceae family prevailed — 104.5 GE per sample on average, only in one case Candida spp. were detected. In 42.1% of cases, when determining TBL, specific types of microorganisms were not identified. Conclusion. On the fetal membranes in full-term pregnancy, the average TBL corresponding to 104.5 (103.5–105.8) GE per sample, in which Enterobacteriaceae prevail in the amount of 104.5 GE per sample on average, is acceptable.