Abstract
The formation processes of denture plaque and denture calculus were observed by optical microscopy and transmission electron microscopy. The distribution of calcium was studied using electron probe X-ray micro analysis (EPMA). Materials were obtained from 3 volunteers who wore an acrylic resin plate for 6 hours, 3 days and 7 days, and 5 complete denture wearers who wore dentures for 5 to 10 years.The denture pellicle was adhered to the denture surface in 6 hours, then the denture plaque was observed3 days later. The denture plaque showed thicker layers and the intermicrobial matrix was formed on the surface in 7 days. Amorphous electron dense deposits appeared in the intermicrobial substances, although no mineral crystals were observed. The lamellated substance (LS) appeared in the denture plaque of the denture patients. The initial mineral crystals of calcium phosphate appeared along the plasma membrane of microorganisms and the lamellated substance and changed into calcification and denture calculus. Consequently, mechanical cleaning and chemical washing were necessary to eliminate the denture plaque and denture calculus. Furthermore, LS of the organic matter below the denture calculus had to be removed by the chemical method.
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