БІОХІМІЧНІ ПОКАЗНИКИ ПУЛУ ОРГАНІЧНОГО КАРБОНУ В НАФТОЗАБРУДНЕНОМУ ҐРУНТІ У ПРОЦЕСІ ФІТОРЕМЕДІАЦІЇ

Abstract

There were investigated the effects of phytoremediation of oil contaminated soil on content of diverse organic Carbon forms in soil. The plants Zea mays L. and Vicia faba var. Minor were used for soil remediation. The experiment lasted for 95 days at all, including the first 22 days without plants. Biochemical and chemical soil properties such as soil microbial biomass (С biom ), soil respiration (V basal ), metabolic quotient (qCO 2 ), carbon availability index (Q r ), total organic Carbon (C оrg ), oil content in soil were used as indicators of soil organic Carbon pool. It was found that soil contamination by oil adversely affected development of tested plants. Oil contamination and plants significantly stimulated С biom and V basal relatively control. Oil presence also caused increase of qCO 2 and Q r , whereasthe effect of plants was insignificant. The increase of Q r in oil contaminated soil reveals lower C deficiency compared to control. The decrease of Q r and qCO 2 in oil contaminated soilduring experimet is a mark of balance recovery in ecosystem. There were determined interaction effects between oil, plants and time by a three way ANOVA. It was shown that effect of plants on С biom та V basal was greater in oil contaminated soil than in the non-polluted soil, and its effect was greater on С biom by the time scale. It indicates the intensification of oil Carbone utilization by microorganisms. Oil contamination caused the increase of soil organic C content in soil relatively to control. After 95 day of experiment content of soil organic C decrease in all experiment series that is related to oil degradation and humus mineralization. The concentration of oil in soil decreased on 45 % during three mouthes of experiment, but effect of plants was insignificant. Thereby oil concentration is not a sensitive indicator of effectiveness of phytoremedy treatment. While biochemical measurements, including С biom та V basal , are more sensitive to detect the effects of phytoremediation of oil contaminated soil.