Abstract
The separation and the quantitative analysis of ATP, ADP, AMP, IMP, inosine and hypoxanthine in the fish muscle were made using the high-performance liquid chromatography with the reversed-phase column.All the ATP related compounds as well as most of the other nucleic acid related compound could be eluted under the phosphate buffer (0.05M-KH2PO4: 0.05M-K2HPO4=1:1) at pH6.78 with the reasonable resolution and the retention time. For the ATP related compounds in the extract of fish muscle, the recovery and the reproducibility by this method were satisfactory and the values obtained agreed with those prepared by the general purpose method of ion exchange chromatography.
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