ヒトへん桃はい中心でのａｐｏｐｔｏｓｉｓ ｔｉｎｇｉｂｌｅ ｂｏｄｙ ｍａｃｒｏｐｈａｇｅを中心に

Abstract

Apoptosis in the human tonsillar germinal center was studied by immunocytochemical methods. It is generally accepted that the germinal center of the lymphoid tissues plays an important role both in cell proliferation and cell death. Although many studies of cell proliferation have been reported, the mechanism of cell death is not yet well understood. According to recent studies, apoptosis indicates programmed cell death, not pathological death, and plays an important role in maintenance, immunity and development of life.In 1885, Flemming reported hematoxin-stained dense bodies within the cell in the germinal center and named them tingible bodies.Subsequent studies showed that the tingible body was a mass of nuclear chromatin from dead cells, and the cell containing tingible bodies was the macrophage, then called the tingible body macrophage.This study investigated the relationship between tingible body macrophages and apoptosis. Tingible body macrophage and adjacent structures were examined by immunostaining. The results were as follows:1. H. E. stained section: tingible body macrophage and tingible bodies were clearly shown. Tingible body was a hematoxin-stained granule.2. Observation of apoptosis: immunostained apoptotic body was shown to be same as tingible body by comparing observations on H. E. stained sections.3. Observation of macrophage : Tingible body macrophage was clearly observed by the CD68 immunostaining method.4. Observation of 5-100 protein positive cell: S-100 protein positive cells consist of large cytoplasma and long cytoplasmic processes. The latter are intermingled with each other to form a network around the tingible body macrophage.5. Observation of B-cells: The immunostained B-cell is found near the tingible body macrophage.6. Observation of T-cells: Immunostained T-cells were found in the germinal center, but did not contact the tingible body macrophage.We discussed apoptosis of the tonsillar germinal center based on the findings cited above.