Abstract
Ficin was covalently immobilized on an acid-soluble matrix of medium (200 kDa) and high molecular weight (350 kDa) chitosans. The enzyme molecules were immobilized in a protein film and copolymerized with glutaraldehyde. The optimal ratio of protein content, total activity and specific activity was observed as a result of ficin covalent immobilization on a matrix of medium-molecular chitosan with 15% glutaraldehyde and high-molecular chitosan with 10% glutaraldehyde. The obtained biocatalysts are promising for industrial applications. ficin, chitosan, glutaraldehyde, covalent immobilization. This work was financially supported by a grant from the President of the Russian Federation for state support to young Russian scientists-doctors of sciences MD-1982.2020.4. Agreement 075-15-2020-325).
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