Abstract
A specific protein which appeared in plasma after a 10km-run was detected using two-dimensional electrophoresis, and it's properties were investigated. This specific protein was detected at almost the same position as albumin on two-dimensional gel under non-denaturing condition, but it was not adsorbed to anti-albumin-Sepharose column. The molecular weight was estimated to be 25, 000 on SDS-polyacrylamide gel electrophresis. This protein was stained with periodic acid-Shiff reagent but not with Sudan Black. This protein was also detected in triathlon race, but it's increase did not definite.
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