口腔へん平上皮癌の臨床的，免疫組織学的検討 Ｓ‐１００陽性細胞について

Abstract

We clinico-pathologically and immunobistochemically studied 80 cases of squamous cell carcinoma of the oral cavity. Of the 80 cases, primary cervical lymph node metastasis was observed in 20 cases and secondary cervical lymph node metastasis in 8 cases. Thc materials were obtained before the treatment, fixed in 10% formalin or 2% PLP, and embedded in paraffin. Immunostaining of S 100 protein was performed by the aviclin-biotin peroxidase complex (ABC) method with polyclonal anti-human S-100 protein antibodies. S-100 proteinpositive cells were graded as “very slight”: less than 2 positive cells, “slight”: 2-5 positive cells, “moderate”: 6-10 positive cells, “marked” more than 11 in the 200X field.Results showed that higher frequency of S-100 protein-positive cells in cases of welldifferentiated cancer region was observed than the peritumoral epithelia. In addition, the grade of S-100 protein-positive cells was related to the density of the lymphocytic infiltration in the primary tumor. Moreover, the number of S-100 protein-positive cells in the metastatic cancer tissues in the lymph nodes was more frequent than in the primary tumor region.Thus, these findings suggested that S-100 protein-positive cells play some immunological roles in squamous cell carcinoma of the oral cavity. However, the present study showed that the presence of cervical lymph node metastasis was not, atisfactorily predicted by the frequency of the 5-100 protein-positive cells.