ДОСЛІДЖЕННЯ КОМПОНЕНТНОГО СКЛАДУ ТА АНТИОКСИДАНТНИХ ВЛАСТИВОСТЕЙ ЕКСТРАКТУ ПРОДУКТУ ПЕРЕРОБКИ ТОМАТА

Abstract

Nowadays in the field of chemical technologies and engineering, research on obtaining ecologically safe phytochemicals / organic compounds from plant raw materials and waste from their processing is gaining more and more development in the vector of “green” technologies. The search of promising sources of phytochemical / organic compounds and the selection of effective “green” extractants is a topical issue for the modern industry of chemical technology and engineering. Recent publications that tomato contains polyphenolic compounds (p-coumaric acid and quercetin derivatives), as well as non-phenolic compounds (benzyl alcohol, saturated and unsaturated fatty acids, carotenoids, etc.) were considered, and extraction processes of these compounds were considered. Information on the use of deep eutectic solvents for the extraction of polyphenolic compounds of tomato pomace. To study the component composition of the tomato processing product extract obtained with a deep eutectic solvent and to establish its antioxidant / antiradical capacity. The efficiency of using a low-temperature eutectic solvent [choline chloride] / [DL-lactic acid] for the extraction of organic compounds from the tomato processing product (Lycopersicon esculentum. Mill.) was investigated. The composition of the extract was studied by high performance liquid chromatography (HPLC). The main components of the extract are p-coumaric and chlorogenic acids, quercet and rutin. The presence of phenolic compounds and flavonoids in the extract was quantified by spectrophotometric method. The phenolic compounds in the test extract were determined using Folin-Chocolteu reagent and expressed as gallic acid equivalent (mgGC / g of extract). The flavonoid content of the extract was determined by the spectrophotometric method with aluminum chloride and expressed in quercetin equivalent (mgKV / g of extract). The antioxidant activity of the obtained extract was also determined by a method using an ethanolic solution of the stable free radical DPPH and a cation radical ABTS. Antiradical activity is expressed as a percentage of inhibition. The work uses an environmentally friendly, ionic solvent for the extraction of organic compounds from tomato pomace. The component composition and antiradical/antioxidant properties of tomato pomace extract obtained by extraction with lowtemperature eutectic solvent based on the choline chloride and lactic acid were studied. Polyphenolics compounds are the main class of extracted compounds. It was found that at concentrations of 60–80 ml/mg extract radical-absorbing efficiency against the radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the cation radical 2,2′-azino-bis-(3-ethylbenzthiazolino)-6-sulfonic acid (ABTS) is at the level of 40 % and 50 %, respectively. Thus, tomato pomace extract obtained by using DES has a high antiradical / antioxidant activity.