دراسة البکتریا المنتجة لانزیم السلیولیز المعزولة من بیئات مختلفة وایجاد الظروف المثلى لانتاج الانزیم.

Abstract

One hundred eighty samples were collected from (soil, insect intestine, leaves, stomach of ruminants, and sewage water) and only five isolates were selected depend on its ability to lyses cellulose and they were one from each isolation source , the selected isolates were belonged to (Staphylococcus sciuri , Streptococcus parasanguinis , Sphingomonaspaucimobilis , Rhizobium radiobacter , Escherichia coli ) with lyses zone (42,28,39,35,32 mm respectively). The PH effect change showed that best activity for cellulase enzyme was 6.627 IU/ml at PH 6 when local isolate Staph. Sciuri was used, and the enzyme activity were 5.857,5.518,5.996 IU/ml for strep. parasanguinis , Sphingo. paucimobilis , Rhizo. Radiobacter respectively at PH 7, while local isolate E. coli gave best cellulase activity at PH 6.5 reached to 4.963 IU/ml. Efficiency of five isolates were varied in their ability of cellulase activity in liquid media under different temperature, best activity of enzyme for local isolates Sphingo. Paucimobilis and Staph. Sciuri 30 C°, while best enzyme activity was recorded at 25C° for local isolates Strep. Parasanguinis and E. coli, and optimum temperature for enzyme production for Rhizo. Radiobacter was 50 C°. Also aeration and agitation effect was studied for their effect on cellulase activity, and the results showed increasing in activity of enzyme at 50 rpm in local isolates Strep. parasanguinis , Sphingo. paucimobilis , Rhizo. Radiobacter and E. coli while the best enzyme activity for Staph. Sciuri was at 100 rpm. Results also shown that the best productivity of enzyme was after two days of incubation for all isolates.