Вплив цистеїл-гістидил-тирозил-гістидил-ізолейцину на вміст продуктів пероксидного окиснення ліпідів та активних форм Оксигену в щурів різного віку за дії важких металів і гліфосату

Abstract

Introduction. Oxidative stress plays a critical role in the pathophysiology of several diseases: liver cirrhosis, atherosclerosis, diabetes mellitus, ischemic heart disease, carcinogenesis, etc. The main mechanism of oxidative stress induced is the disturbance oxidation and reduction processes by various factors such as toxic substances, radiation, temperature, drugs. Ions of heavy metals (transition metals) lead to the production of reactive oxygen species (ROS), causing a lipid peroxidation and other main biomolecules (proteins, nucleic acids).The aim of the study – to learn the influence of peptid: cisteil-histidil-tyrosil-histidil-isoleucine on generation of active forms of oxygen the content of products of free lipid peroxidation.Research Methods. The experiment was carried out on lab nonlinear white rats – males three age periods: puberty, mature and old aging animals. The rats received the lead acetate, copper sulfate, glyphosate (in herbicide Roundup) in combined and peptide as correction agent. Subhronic lesions in rats was modelled by intragastric administration of water solution of Lead Acetate at a dose of 11 mg/kg, Copper Sulfate at a dose of 13 mg / kg, Glyphosate at a dose of 250 mg / kg. Dehlorinated drinking tap water to intact animals was added. The correction effect was research by the during 10 days intragastric administration of water solution of peptide cisteil-histidil-tyrosil- histidil-isoleucine in a dose of 9 mg / kg at 21th day of the experiment after 6 hours after toxins. The degree of lipid peroxidation was assayed by estimating the thiobarbituric acid reactive substances (TBARS) and diene conjugates (DK) in blood serum and liver homogenates by spectrophotometric method. The superoxide anion-radical content was determined by test with Nitrobluetetrazolium, hydroxyl radical content was determined by the product of oxidation of 2-deoxyribose.Results and Discussion. It was established that the production of reactive oxygen species and lipid peroxidation increase by the combined effect of glyphosate (in Roundup form herbicide), lead acetate, copper sulfate in different age rats. There was a significant increase in concentration of TBARS, diene conjugates, superoxide anionradical and hydroxyl radical. One of the most probable mechanisms of peptide: cisteil-histidil-tyrosil-histidil-isoleucine corrective activity is based on its ability to antioxidantion activity that decrease concentration of a production of reactive oxygen species and a lipid peroxidation.Conclusion. The protective effect of peptide: tsysteil-histidil-tyrosil-histidil-isoleucine on liver damages rats induced by toxicity is administration induced loss to the production of reactive oxygen species, causing a lipid peroxidation