Abstract
To investigate molecular and cellular biology of deep-sea multicellular organisms, the author has developed a novel piezo-stat aquarium system (Deep Aquarium System). Using this system, the author succeeded in maintaining a variety of deep-sea multicellular organisms under pressure and under atmospheric conditions after gradual and slow decompression. Furthermore, the author successfully cultivated pectoral fin cells of a living deep-sea fish that decompressed to atmospheric pressure. The deep-sea fish cells passaged more than 20 times and were succeeded in freeze storage under atmospheric condition. The deep-sea fish cells have greater pressure tolerance abilities than conger eel and mouse cells.
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