新しい構造脂質の調整（原標題は英語）

Abstract

Structured lipids with n-6/n-3 polyunsaturated fatty acids (PUFA) or medium chain fatty acids (MC) at optimal ratios were prepared by lipase catalyzed transacylation of tuna orbital oil consisting of about 40% n-3 PUFA such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) with purified linoleic acid or MC, such as caprylic, capric, and lauric acids, at 60 °C. 1, 3- Specific immobilized lipases of Lipozyme TL-IM and Lipozyme RM-IM originated from Thermomyces lanuginosus and Rhizomucor miehei were used in the preparation, respectively. By controlling enzyme amount from 20-80 IUN for Lipozyme TL-IM and 1-12 BAUN for Lipozyme RM-IM with reaction time from 4 to 24h, structured lipids at n-6/n-3 ratios from 1.3 till 6.9 were easily prepared by the reaction of tuna orbital oil with highly purified linoleic acid. MC was introduced into tuna orbital oil at various MC/n-3 PUFA ratios such as 0.67-1.89 for caprylic, 0.73-2.85 for capric, and 0.91-4.08 for lauric acids, respectively during transacylation for 32 h. Lipozyme RM-IM was clearly shown a better enzyme for the present transacylation, compared to Lipozyme TL-IM in consideration of the above ratio values and triglycerides recovery.