Abstract
We studied the fibrinolytic activity, amount and localization of plasminogen activator (PA) in bovine vitreous extract (VE) and aqueous humor (AH). Fibrinolytic activity of VE and AH was quenched by adding anti-human t-PA IgG, but not by anti-human HMW u-PA IgG. After SDS electrophoretic analysis, enzymographic distributions of PA in VE and AH were detected at about Mr. 66K and 110K. These findings suggested the fact that the main PA in VE and AH was t-PA. Estimating fibrinolytic activity of VE and AH by amidolytic assay, activity was 0.12±0. 10IU/ml (n=10) in VE and 0.04±0.07IU/ml (n=10) in AH. Measuring the amount of t-PA by RIA, t-PA was 4.1±0.9ng/ml (n=10) in VE, and 3.3±0.8ng/ml in AH. Investigating the PA activity by Todd's fibrinolysis autography, clear lytic areas could be seen on the small vessels of the iris, and the subepithelial stroma and along the endothelium of the cornea. In immunohistochemical studies using anti-human t-PA IgG, vascular endothelial cells and corneal epithelial cells showed the positive reactivity.
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