Abstract
A simple biosensor for measuring sodium channel blockers, such as tetrodotoxin (TTX) and saxitoxin (STX), and paralytic shellfish poisoning (PSP) consisted of frog bladder membrane, and Na+ electrode. Effects of storage temperature, antiseptic and species of frog on the activity of Na+ channel on the membrane were investigated from the standpoint of practical use.The results obtained were as follows:1) The maximum output of the sensor was obtained at 30 to 33°C and pH 4.8 to 6.0.These values were shown higher in the case of tropical frog than a temperate frog. This was assumed due to the effect of living the environment.2) Frog bladder was able to keep at 5°C during 7 months, but at -30°C during only 80 days.3) Antiseptic effect of NaN3 on the frog bladder membrane was recognized in the concentration of 0.003%.
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