Abstract
The technique of obtainment of a new bioplastic material based on collagen, elastin and hyaluronic acid is described. The results of a study of the biomaterial structure and properties to assess the prospects for its further use in clinical practice are also presented. To prepare the material samples, collagen, elastin and hyaluronic acid were mixed in predetermined proportions with distilled water, and the resulting biofilm was crosslinked by UV irradiation. A commercial human skin fibroblast cell culture (HDF) was used to assess the biomaterial cytotoxicity and biocompatibility; as a result, it was shown that cytotoxicity is absent in it. The visualization and studies of the biomaterial structure were carried out using light microscopy. The new material was shown to be highly elastic and resilient; it also had a high porosity with a pore diameter of 100-200 um. It was shown that the biomaterial samples were able to maintain physical properties in the culture medium for more than 10 days, while the complete destruction of the matrix was observed 3-4 weeks after the beginning of incubation. Due to its physical properties and structure, and also the capacity of providing effective conditions for good cell proliferation, the created biomaterial can be used as a wound cover in the damaged skin areas. This allows us to consider the new biomaterial promising for clinical practice. cell technologies, tissue-engineered constructs, bioplastic material, collagen, human fibroblasts.
Published Version
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