Гістоморфологічне обґрунтування ілеотрансверзоанастомозу в експерименті

Abstract

Advantages of the suggested end-to-side ileotransverseanastomosis in the view of comparatively better course of compensatory processes and quick restoration of normal functional capacity of the bowels are histomorphologically substantiated in this paper. The aim of the research – to determine histomorphologically the state of the mucous membrane of the large and small intestine depending on the method of ileotransverseanastomosis formation following resection of ileocecal angle. Material and methods. Experiment was carried out on 19 mongrel dogs, who underevent resection of ileocecal angle. The animals were devided into two groups; basic and group of comparison group. Ileotransverseanastomosis was formed to the group of comparison according to the methods of Kimbarovsky and O.I. Ivashchuk (1997) and the own method was used (pat. № 85715) – to the basic group. Sampling of the material was carried out on the 7th, 15th, and 30th day after operative intervention, as well as during anastomosis formation. Histomorphological anastomosis of the mucous membrane of the large and small intestines was carried out. Discussion of the results. Resection of ileocecal angle results in probable lowering of optical density of PAS-positive glycocalyx staining, PAS-positive staining of the goblet cells and the number of the latters as all. The obtained results of nvestigation show probably higher indices in the basic group of animals against the group of comparison during the period of observation. Optic density of PAS-positive glycocalyx staining and PAS- positive staining of goblet cells of the mucose membrane of the large intestine on the 30 th day has incredible difference in comparison wit indices before operation. Conclusion. Formation of the suggested large – small intestinal anastomosis after removing ileocecal angle is characterized by higher optic density of PAS-positive glycocalyx staining, PAS-positive staining of goblet cells as well as the quantity of the latters in comparison with their nearest analogues that shows better course of compensatory processes and rapid restoration of the normal functional capacity of the bowels.