Abstract

본 실험은 표고 톱밥재배에서 배지 부영양원 종류와 버섯발생 온도에 따라 버섯의 아미노산 함량 변화를 분석하였다. 배지는 톱밥과 부영양원(미강, 보리, 콩비지, 홍삼박)의 비율이 8:2에 칼슘 0.5% 첨가와 미첨가 등 8처리구로 하였다. 버섯의 발생온도는 저온(<TEX>$13{\sim}15^{\circ}C$</TEX>), 중온(<TEX>$18{\sim}20^{\circ}C$</TEX>), 고온(<TEX>$24{\sim}26^{\circ}C$</TEX>)으로 처리하였다. 아미노산은 모든 처리구에 17종이 검출되었고 영양원 종류에 따라 성분차이들이 나타났다. 총아미노산 함량은 홍삼박 배지에서 209.7 mg/g으로 가장 높았고, 가장 많이 검출된 아미노산은 글루탐산이었고 보리배지에 51.3 mg/g 이었다. 버섯발생 온도처리 조건에서 총아미노산 함량은 중온에서 38.2%, 저온에서 32.5%, 고온에서 29.3% 순으로 나타났고, 중온과 저온 조건에서 아미노산 함량 순위는 비슷한 경향을 나타냈다. 본 연구 결과는 표고의 맛과 기능성을 갖는 아미노산의 종류를 제시하는 기초자료로 활용될 수 있을 것으로 기대한다. In this study, we investigated the amino acids content of shiitake mushrooms grown in eight different media and under three different temperature conditions. The sawdust media were supplemented with 4 kinds of nutrients - rice bran, barley, bean curd refuse, and red ginseng refuse in an 8:2 ratio with or without 0.5% calcium chloride, and the fruiting temperature was set to low (<TEX>$13-15^{\circ}C$</TEX>), medium (<TEX>$18-20^{\circ}C$</TEX>), or high temperatures (<TEX>$24-26^{\circ}C$</TEX>). Seventeen amino acids were detected, and the content varied depending on the compositions of the media. The highest total amino acid content (209.7 mg/g) was measured in mushrooms from the media with red ginseng refuse, and the glutamic acid content was the highest (51.3 mg/g) in mushrooms from the media with barley. In terms of fruiting temperature, the amino acid content was the highest (38.2%) at medium temperature followed by the content at low (32.5%) and high temperature (29.3%). The amino acid composition of shiitake mushrooms was similar between the medium and low temperature conditions.

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