ХАРАКТЕРИСТИКА ЖИЗНЕСПОСОБНОСТИ КЛЕТОК ЛИПОГРАФТА ПРИ РАЗЛИЧНЫХ МЕТОДИКАХ ЕГО ПОЛУЧЕНИЯ И ПОДГОТОВКИ

Abstract

Objective. To determine the predictors of adipocytes and fibroblast-like cells survival in the lithographt using various techniques of lipoaspiration and adipose tissue preparation to autotraslantation. Methods. Cellular viability was analyzed in 57 adipose tissue samples prepared by various techniques of lipoaspiration, and analysis of damage to adipocytes and fibroblast-like cells - in 73 smears after passing of adipose tissue through filters of different diameters. Results. The average adipocyte viability in untreated lipoaspirate was 59%. Using water-jet technique, it was 65% (median 61%), syringe technique - 65% (median 74%), suction-assisted lipectomy - 55% (median 44%), p=0.18. The number of viable adipocytes depending on donor sites: thighs - 76%, waist - 67%, abdomen - 57%, buttocks - 50%, shoulders - 38%, knees - 35%. The number of intact adipocytes after 1.4 mm filter processing was 62-68%, fibroblast-like cells - 24-28%. The number of viable cells reduced with each passage. The number of intact adipocytes after 1.2 mm filter was 42-52%; of non-damaged fibroblast-like cells were 24-26% which located among connective tissue. The number of intact cells after emulsifying filter elevated up to 4-16% of adipocytes and 6-16% of fibroblast-like cells with a reduction of viable cells number with increasing of each passage. Single connective tissue fibers were determined, most content of the smear was represented by homogeneous fat. Conclusion. The viability of adipocytes is higher when using the syringe lipoaspiration technique with sampling from the inner and outer surfaces of the thighs and lower back. For regenerative purposes, it is preferable to use emulsified fat, characterized by the destruction of adipocytes and the elimination of connective tissue fibers, keeping intact up to 16% of fibroblast-like cells. The defects of soft tissue is better to fill with washed «macro-fat» without filtration, or use a 1.4 mm cell filter. For a combination of regenerative and volumizing purposes, it is advisable to use anaerobic cell filters. What this paper adds For the first time the viability of adipocytes using different lipoaspiration techniques and taken from different anatomical areas has been analyzed. An original technique for analyzing the viability of lipograft has been firstly applied. A reliable difference in the cellular composition of the lipograft when using different cell filters has been found. A reliable significant negative relationship between the number of viable cells and the number of passages through cell filters (D-1.4, 0.5 mm.) has been established. Recommendations for the preparation of various types of lipograft to obtain the maximum ratio of viable cells were substantiated.