Abstract

The purpose of the work was to determine the content of Calcium and Phosphorus in bone tissue, as well as the tibia strength index of broiler chickens under using two feed additives made from the waste of primary processing of marine hydrobionts in the diet. The experiment was carried out on “Ross 308” cross broilers, which were divided into 5 groups: 1 control and 4 experimental. Each additive was fed in two ways: by replacing 7% of the diet weight and adding 7% to it. Chickens of the control group were fed only the basic diet (BD), balanced by basic nutrients. Chickens of experimental groups from 20 to 42 days old were additionally fed the feed additives. Chickens D-1 and D-2 groups consumed a mineral feed additive (MFA), D-3 and D-4 groups received a protein-mineral feed additive (PMFA). Moreover, broilers of D-1 and D-3 groups by feed weight received 93% of BD and 7% of MFA and PMFA respectively, D-2 and D-4 groups in addition to BD (100%) received 7% respectively of MFA and PMFA. MFA is milled leaves of the Black Sea Mussel (Mytilus galloprovincialis) and seawater. PMFA contained milled leaves of large and the body of small mussels, waste from the primary processing of red algae Phyllophora nervosa and seawater. As a material for research was considered the chickens’ tibia of of 42 days old. The concentration of total Calcium and inorganic Phosphorus was determined in the middle part of the tibia using a UV mini-1240 SHIMADZU spectrophotometer (Japan) and a set of reagents NPP Filisit-Diagnostika (Ukraine). The bone cross-sectional area was determined by scanning prior to testing with processing in the Compass-3D graphics program. The mechanical parameters of the bones were determined on an FM-1000 testing machine under the action of a double-support bend. The use of feed additives contributed to an increase in the content of Calcium and Phosphorus in the bone tissue of broiler chickens and, accordingly, the strength of the tibia. The Phosphorus content increase took place to a greater extent than Calcium, which caused a decrease in the Calcium-Phosphorus ratio. The content of Calcium and Phosphorus in the bone tissue, as well as the strength of the tibia of the chickens increased due to diet enrichment with feed additives, and not its partial replacement.

Highlights

  • The condition of the bone tissue of broiler chickens when using feed additives from marine hydrobionts

  • The purpose of the work was to determine the content of Calcium and Phosphorus in bone tissue, as well as the tibia strength index of broiler chickens under using two feed additives made from the waste of primary processing of marine hydrobionts in the diet

  • The concentration of total Calcium and inorganic Phosphorus was determined in the middle part of the tibia using a UV mini-1240 SHIMADZU spectrophotometer (Japan) and a set of reagents NPP Filisit-Diagnostika (Ukraine)

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Summary

Introduction

The condition of the bone tissue of broiler chickens when using feed additives from marine hydrobionts. The purpose of the work was to determine the content of Calcium and Phosphorus in bone tissue, as well as the tibia strength index of broiler chickens under using two feed additives made from the waste of primary processing of marine hydrobionts in the diet. The use of feed additives contributed to an increase in the content of Calcium and Phosphorus in the bone tissue of broiler chickens and, the strength of the tibia.

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