잎새버섯 균주의 균사체 생육 최적조건

Abstract

잎새버섯의 우량계통을 선발하여 농가에 보급하기 위하여 수집균주들의 균사체 생육 최적조건을 검토한 결과는 다음과 같다. 잎새버섯 수집균주의 생장에 적합한 배지는 PDA배지가 선발되었으며, 균사배양 적온은 <TEX>$25^{\circ}C$</TEX>로 나타났다. 잎새버섯의 균사생장에 적합한 수소이온 농도는 pH <TEX>$4{\sim}5$</TEX>로 약산성에서 균사생장이 우수하였다. 균사생장에 적합한 탄소원으로는 glucose와 fructose, 질소원으로는 peptone이 우수하였으며 C/N율은 <TEX>$10{\sim}20$</TEX>으로 질소 요구도가 다소 높은 것으로 나타났다. This study was carried out to obtain basic data on mycelial growth characteristics for an artificial cultivation of Grifola frondosa. Ten strains of G. frondosa were collected from Korea, China and Japan and investigated its optimal culture condition. Among four kinds of mushroom culture media, PDA medium was selected as the suitable culture medium. The optimal conditions for the mycelial growth of G. frondosa in PDA medium were <TEX>$25^{\circ}C$</TEX> and <TEX>$4{\sim}5$</TEX> of pH, respectively. The carbon and nitrogen sources for the optimum mycelial growth were fructose and peptone, respectively, and the highest mycelial growth was observed when the C/N ratio was <TEX>$10{\sim}20$</TEX>.