ＣＯＢＥ Ｓｐｅｃｔｒａによる濃厚血小板採取の検討

Abstract

With the increase in demand for platelet concentrate (PC), how to meet it and side effects due to WBC mixed in platelet preparations have become great problems. We studied a total of eighty four platelet donations done by the donors (male 80, female 4), who met all the criteria for platelet donation, during the period of April 1990 to August 1991.1) According to the initial count of platelets, 28 donors were divided into Group A (less than 25×104cells/μl), 22 into Group B (25×104/μl to 30×104/μl), 22 into Group C (30×104/μl to 35×104/μl) and 12 into Group D (more than 35×104/μl). The collection rate of PC of more than 15 units (3.0×1011) was 25.0%, 50.0%, 81.8% and 91.7% in respective group.2) As for the time-course change in concentration of platelets collected, it was 122.3±19.0×104cells/μl in Group A 30 min after the start of collection and 177.8±32.6×104cells/μl in Group B and C 50 min after the start; these were the, highest values in each group.3) The average count of WBC mixed in was as few as 3cells/μl and 6.9×105cells/bag.4) As for the time-course change in count of WBC mixed in, it was highest at the start of collection, 14.3±14.5cells/μl, and thereafter decreased in the course of time.5) We tried to take photos of the interface forming process in centrifuge channel. Whole blood flow rate was maintained at 40ml/min up to 500ml blood volume processed to obtain proper interface, since the establishment of interface directly affects platelet collecting efficiency and white blood cell contamination.6) The volume of citric acid injected was 21.9mg/TBV-1/min on an average and 29.4mg/TBV-1/min at the maximum, causing no side effects, and the Ca++ reduction was only 20.8%.